Enzyme Kinetics - Temperature
The Experiments
The effect of temperature on the rate of enzyme action will be determined by performing four experiments:
1. Two ml of catalase solution, mixed with two ml of H2O2 at 0oC.
2. Two ml of catalase solution, mixed with two ml of H2O2 at 25oC (room temp).
3. Two ml of catalase solution, mixed with two ml of H2O2 at 37oC (body temp).
4. Two ml of catalase solution, mixed with two ml of H2O2 at 55oC.
Notice that the only experimental variable being changed is the temperature at which the reaction is run.
Results
Using the data below prepare a graph with temperature as the independent variable (x-axis) and rate of O2 formation in mls/sec as the dependent variable (y-axis). From this graph determine the optimum temperature for this enzyme.
experiment
#
| temperature
(oC)
| see results
|
| 1
| 0
| 
|
| 2
| 25
|
|
| 3
| 37
|
|
| 4
| 60
|
|
