Enzyme Kinetics - Substrate Concentration
Effect of Substrate Concentration
|
The Experiments
The effect of substrate concentration on the rate of enzyme action will be determined by performing four experiments:
1. Two ml of catalase solution mixed with 0.5 ml of H2O2 and 1.5 ml of water at 25oC.
2. Two ml of catalase solution mixed with 1.0 ml of H2O2 and 1.0 ml of water at 25oC.
3. Two ml of catalase solution mixed with 1.5 ml of H2O2 and 0.5 ml of water at 25oC.
4. Two ml of catalase solution mixed with 2.0 ml of H2O2 and 0.0 ml of water at 25oC.
Notice that the only experimental variable being changed is the concentration of the substrate being used. The water is added to make sure that total volume of the solution remains the same.
Results
Using the data below prepare a graph with substrate concentration as the independent variable (x-axis) and rate of O2 formation in mls/sec as the dependent variable (y-axis). From this graph describe the effect of substrate concentration on rate of reaction.
experiment
#
| H2O2 solution
(mls)
| see results
|
| 1
| 0.5
| 
|
| 2
| 1.0
|
|
| 3
| 1.5
|
|
| 4
| 2.0
|
|
